This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wiley, W. R.
Right arrow Articles by Stokes, J. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wiley, W. R.
Right arrow Articles by Stokes, J. L.

 Previous Article  |  Next Article 

J Bacteriol. 1962 October; 84(4): 730-734
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.

REQUIREMENT OF AN ALKALINE pH AND AMMONIA FOR SUBSTRATE OXIDATION BY BACILLUS PASTEURII

W. R. Wiley and J. L. Stokes

Department of Bacteriology and Public Health, Washington State University, Pullman, Washington

ABSTRACT

WILEY, W. R. (Washington State University, Pullman) AND J. L. STOKES. Requirement of an alkaline pH and ammonia for substrate oxidation by Bacillus pasteurii. J. Bacteriol. 84:730–734. 1962—Resting-cell suspensions of Bacillus paseurii require a pH higher than 8.0 and also NH4+ for the oxidation of glycine, alanine, serine, glutamic acid, other amino acids, and compounds of the Krebs cycle. The optimal pH is 9.2. Maximal activity was obtained with 0.5% (NH4)2SO4, the highest concentration tested. NH4+ can be supplied by a number of inorganic and organic ammonium salts. The effect of NH4+ is fairly specific. It cannot be replaced by Na+, Cs+, and Mg++, and only partially replaced by Li+, K+, Rb+, and Ca++. Some possible metabolic functions of the required alkaline pH and NH4+ are discussed.

J Bacteriol. 1962 October; 84(4): 730-734
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»