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Journal of Bacteriology, November 2005, p. 7857-7862, Vol. 187, No. 22
0021-9193/05/$08.00+0     doi:10.1128/JB.187.22.7857-7862.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Actin-Binding Proteins from Burkholderia mallei and Burkholderia thailandensis Can Functionally Compensate for the Actin-Based Motility Defect of a Burkholderia pseudomallei bimA Mutant

Joanne M. Stevens,1 Ricky L. Ulrich,2 Lowrie A. Taylor,1 Michael W. Wood,1 David DeShazer,2 Mark P. Stevens,1 and Edouard E. Galyov1*

Division of Microbiology, Institute for Animal Health, Compton Laboratory, Berkshire RG20 7NN, United Kingdom,1 Bacteriology Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland2

Received 1 June 2005/ Accepted 23 August 2005

Recently we identified a bacterial factor (BimA) required for actin-based motility of Burkholderia pseudomallei. Here we report that Burkholderia mallei and Burkholderia thailandensis are capable of actin-based motility in J774.2 cells and that BimA homologs of these bacteria can restore the actin-based motility defect of a B. pseudomallei bimA mutant. While the BimA homologs differ in their amino-terminal sequence, they interact directly with actin in vitro and vary in their ability to bind Arp3.

* Corresponding author. Mailing address: Division of Microbiology, Institute for Animal Health, Compton Laboratory, Berkshire RG20 7NN, United Kingdom. Phone: 44 1635 577291. Fax: 44 1635 577243. E-mail: edouard.galyov{at}bbsrc.ac.uk.

Journal of Bacteriology, November 2005, p. 7857-7862, Vol. 187, No. 22
0021-9193/05/$08.00+0     doi:10.1128/JB.187.22.7857-7862.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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