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J. Bacteriol., 11 1997, 7156-7160, Vol 179, No. 22
Copyright © 1997, American Society for Microbiology

Designing recombinant Pseudomonas strains to enhance biodesulfurization

ME Gallardo, A Ferrandez, V De Lorenzo, JL Garcia and E Diaz
Departamento de Microbiologia Molecular, Centro de Investigaciones Biologicas, Consejo Superior de Investigaciones Cientificas, Madrid, Spain.

The dsz biodesulfurization cluster from Rhodococcus erythropolis IGTS8 has been engineered under the control of heterologous broad-host-range regulatory signals to alleviate the mechanism of sulfur repression, and it was stably inserted into the chromosomes of different Pseudomonas strains. The recombinant bacteria were able to desulfurize dibenzothiophene more efficiently than the native host. Furthermore, these new biocatalysts combine relevant industrial and environmental traits, such as production of biosurfactants, with the enhanced biodesulfurization phenotype.

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