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J. Bacteriol., 10 1997, 6228-6237, Vol 179, No. 20
Copyright © 1997, American Society for Microbiology

Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization

AJ Link, D Phillips and GM Church
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.

We have developed a new system of chromosomal mutagenesis in order to study the functions of uncharacterized open reading frames (ORFs) in wild-type Escherichia coli. Because of the operon structure of this organism, traditional methods such as insertional mutagenesis run the risk of introducing polar effects on downstream genes or creating secondary mutations elsewhere in the genome. Our system uses crossover PCR to create in-frame, tagged deletions in chromosomal DNA. These deletions are placed in the E. coli chromosome by using plasmid pKO3, a gene replacement vector that contains a temperature-sensitive origin of replication and markers for positive and negative selection for chromosomal integration and excision. Using kanamycin resistance (Kn(r)) insertional alleles of the essential genes pepM and rpsB cloned into the replacement vector, we calibrated the system for the expected results when essential genes are deleted. Two poorly understood genes, hdeA and yjbJ, encoding highly abundant proteins were selected as targets for this approach. When the system was used to replace chromosomal hdeA with insertional alleles, we observed vastly different results that were dependent on the exact nature of the insertions. When a Kn(r) gene was inserted into hdeA at two different locations and orientations, both essential and nonessential phenotypes were seen. Using PCR-generated deletions, we were able to make in-frame deletion strains of both hdeA and yjbJ. The two genes proved to be nonessential in both rich and glucose-minimal media. In competition experiments using isogenic strains, the strain with the insertional allele of yjbJ showed growth rates different from those of the strain with the deletion allele of yjbJ. These results illustrate that in-frame, unmarked deletions are among the most reliable types of mutations available for wild-type E. coli. Because these strains are isogenic with the exception of their deleted ORFs, they may be used in competition with one another to reveal phenotypes not apparent when cultured singly.


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  • Grass, G., Franke, S., Taudte, N., Nies, D. H., Kucharski, L. M., Maguire, M. E., Rensing, C. (2005). The Metal Permease ZupT from Escherichia coli Is a Transporter with a Broad Substrate Spectrum. J. Bacteriol. 187: 1604-1611 [Abstract] [Full Text]  
  • Fuller-Schaefer, C. A., Kadner, R. J. (2005). Multiple Extracellular Loops Contribute to Substrate Binding and Transport by the Escherichia coli Cobalamin Transporter BtuB. J. Bacteriol. 187: 1732-1739 [Abstract] [Full Text]  
  • Crozat, E., Philippe, N., Lenski, R. E., Geiselmann, J., Schneider, D. (2005). Long-Term Experimental Evolution in Escherichia coli. XII. DNA Topology as a Key Target of Selection. Genetics 169: 523-532 [Abstract] [Full Text]  
  • Nakamaru-Ogiso, E., Yano, T., Yagi, T., Ohnishi, T. (2005). Characterization of the Iron-Sulfur Cluster N7 (N1c) in the Subunit NuoG of the Proton-translocating NADH-quinone Oxidoreductase from Escherichia coli. J. Biol. Chem. 280: 301-307 [Abstract] [Full Text]  
  • Otsuka, Y., Yonesaki, T. (2005). A Novel Endoribonuclease, RNase LS, in Escherichia coli. Genetics 169: 13-20 [Abstract] [Full Text]  
  • Collier, J., Bohn, C., Bouloc, P. (2004). SsrA Tagging of Escherichia coli SecM at Its Translation Arrest Sequence. J. Biol. Chem. 279: 54193-54201 [Abstract] [Full Text]  
  • Wan, X.-F., VerBerkmoes, N. C., McCue, L. A., Stanek, D., Connelly, H., Hauser, L. J., Wu, L., Liu, X., Yan, T., Leaphart, A., Hettich, R. L., Zhou, J., Thompson, D. K. (2004). Transcriptomic and Proteomic Characterization of the Fur Modulon in the Metal-Reducing Bacterium Shewanella oneidensis. J. Bacteriol. 186: 8385-8400 [Abstract] [Full Text]  
  • Vijayakumar, S. R. V., Kirchhof, M. G., Patten, C. L., Schellhorn, H. E. (2004). RpoS-Regulated Genes of Escherichia coli Identified by Random lacZ Fusion Mutagenesis. J. Bacteriol. 186: 8499-8507 [Abstract] [Full Text]  
  • Netzer, R., Peters-Wendisch, P., Eggeling, L., Sahm, H. (2004). Cometabolism of a Nongrowth Substrate: L-Serine Utilization by Corynebacterium glutamicum. Appl. Environ. Microbiol. 70: 7148-7155 [Abstract] [Full Text]  
  • Arnaud, M., Chastanet, A., Debarbouille, M. (2004). New Vector for Efficient Allelic Replacement in Naturally Nontransformable, Low-GC-Content, Gram-Positive Bacteria. Appl. Environ. Microbiol. 70: 6887-6891 [Abstract] [Full Text]  
  • Himeno, H., Hanawa-Suetsugu, K., Kimura, T., Takagi, K., Sugiyama, W., Shirata, S., Mikami, T., Odagiri, F., Osanai, Y., Watanabe, D., Goto, S., Kalachnyuk, L., Ushida, C., Muto, A. (2004). A novel GTPase activated by the small subunit of ribosome. Nucleic Acids Res 32: 5303-5309 [Abstract] [Full Text]  
  • Malpica, R., Franco, B., Rodriguez, C., Kwon, O., Georgellis, D. (2004). Identification of a quinone-sensitive redox switch in the ArcB sensor kinase. Proc. Natl. Acad. Sci. USA 101: 13318-13323 [Abstract] [Full Text]  
  • Hoang, L., Fredrick, K., Noller, H. F. (2004). Creating ribosomes with an all-RNA 30S subunit P site. Proc. Natl. Acad. Sci. USA 101: 12439-12443 [Abstract] [Full Text]  
  • Clarke, B. R., Cuthbertson, L., Whitfield, C. (2004). Nonreducing Terminal Modifications Determine the Chain Length of Polymannose O Antigens of Escherichia coli and Couple Chain Termination to Polymer Export via an ATP-binding Cassette Transporter. J. Biol. Chem. 279: 35709-35718 [Abstract] [Full Text]  


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