JB Accepts, published online ahead of print on 30 October 2009

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J. Bacteriol. doi:10.1128/JB.01121-09
Copyright (c) 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Isolation of basal bodies with C-ring components from the Na⁺-driven flagellar motor of Vibrio alginolyticus

Masafumi Koike, Hiroyuki Terashima, Seiji Kojima, and Michio Homma^*

Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan

^* To whom correspondence should be addressed. Email: g44416a{at}cc.nagoya-u.ac.jp.

To investigate the Na⁺-driven flagellar motor of Vibrio alginolyticus, we attempted to isolate its C-ring structure. FliG but not FliM co-purified with the basal bodies. FliM proteins may be easily dissociated from the basal body. We could detect FliG on the MS-ring surface of the basal bodies.