JB Accepts, published online ahead of print on 23 October 2009

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J. Bacteriol. doi:10.1128/JB.00822-09
Copyright (c) 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Molecular basis for the role of Staphylococcus aureus Penicillin Binding Protein 4 in antimicrobial resistance

Vikas Navratna, Savitha Nadig, Varun Sood, K. Prasad, Gayathri Arakere, and B. Gopal^*

Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India; and Sir Dorabji Tata Center for Research in Tropical Diseases, Indian Institute of Science Campus, Bangalore 560 012, India

^* To whom correspondence should be addressed. Email: bgopal{at}mbu.iisc.ernet.in.

Penicillin binding proteins (PBPs) are membrane-associated proteins that catalyze the final step of murein biosynthesis. These proteins function either as transpeptidases or carboxypeptidases and in a few cases demonstrate transglycosylase activity. Both transpeptidase and carboxypeptidase activities of PBPs occur at the D-ala-D-ala terminus of a murein precursor containing the disaccharide pentapeptides N-acetylglucosamine and N-acetyl-muramicacid-L-ala-D-glu-L-lys-D-ala-D-ala. -lactam antibiotics inhibit these enzymes by competing with the pentapeptide precursor for binding to the active site of the enzyme. Here we describe the crystal structure, biochemical characteristics and the expression profile of PBP4, a Low Molecular Mass (LMM) PBP from Staphylococcus aureus strain COL. The crystal structures of PBP4-antibiotic complexes reported here were determined by Molecular Replacement using the atomic coordinates deposited by the New York Structural Genomics consortium. While the pbp4 gene is not essential for the viability of S. aureus, the knockout phenotype of this gene is characterized by a marked reduction in cross-linked muropeptide and increased vancomycin resistance. Unlike other PBPs, we note that expression of PBP4 is not substantially altered in different experimental conditions nor does it change across representative hospital or community associated strains of S. aureus that were examined. In vitro data on purified recombinant S. aureus PBP4 suggests that it is a lactamase and is not trapped as an acyl intermediate with -lactam antibiotics. Put together, the expression analysis and biochemical features of PBP4 provide a framework to understand the function of this protein in S. aureus and its role in antimicrobial resistance.