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J Bacteriol. 1966 April; 91(4): 1509-1515
Copyright © 1966 American Society for Microbiology. All Rights Reserved.

Mutation Leading to Increased Sensitivity to Chromium in Salmonella typhimurium

Division of Biochemistry, Walter Reed Army Institute of Research, Washington, D.C.
Cold Spring Harbor Laboratory of Quantitative Biology, Cold Spring Harbor, New York

ABSTRACT

CORWIN, L. M. (Walter Reed Army Institute of Research, Washington, D.C.), G. R. FANNING, F. FELDMAN, AND P. MARGOLIN. Mutation leading to increased sensitivity to chromium in Salmonella typhimurium. J. Bacteriol. 91:1509–1515. 1966.—Certain deletion mutants including the tryptophan operon in Salmonella typhimurium are unable to utilize several sugars as carbon sources in solid media, although they are able to grow in liquid media with these sugars. The addition of citrate or washing the agar with ethylenediaminetetraacetic acid permits growth on solid media. Analysis of the agar revealed that Fe³⁺ and Cr³⁺ were present at concentrations of 22 and 75 µM, respectively. The addition of Fe³⁺ to liquid media in 0.5 mM concentrations did not inhibit the wild type or the mutants. A similar concentration of Cr³⁺ did not inhibit the wild type, but concentrations as low as 0.01 to 0.05 mM inhibited the deletion mutants. Other metals were inhibitory at various concentrations, but none showed any significant differential effects on the mutants and the wild type. The increased sensitivity of the mutants to chromium may be due either to an increased permeability to Cr³⁺, resulting in higher effective intracellular concentrations and inhibition of one or more metabolic functions, or to a binding of Cr³⁺ to an altered cell wall, resulting in decreased permeability of required substrates.

This article has been cited by other articles:

• Sonerville, R. L. (1966). Tryptophan Operon of Escherichia coli: Regulatory Behavior in Salmonella typhimurium Cytoplasm. Science 154: 1585-1587 [Abstract]