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Journal of Bacteriology, November 2009, p. 7039-7049, Vol. 191, No. 22
0021-9193/09/$08.00+0     doi:10.1128/JB.00897-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

An Interactive Regulatory Network Controls Stress Response in Bifidobacterium breve UCC2003{triangledown} ,{dagger}

Aldert Zomer, Matilde Fernandez,{ddagger} Breda Kearney, Gerald F. Fitzgerald, Marco Ventura,§ and Douwe van Sinderen*

Alimentary Pharmabiotic Centre and Department of Microbiology, National University of Ireland, Cork, Ireland

Received 9 July 2009/ Accepted 28 August 2009

Members of the genus Bifidobacterium are gram-positive bacteria that commonly are found in the gastrointestinal tract (GIT) of mammals, including humans. Because of their perceived probiotic properties, they frequently are incorporated as functional ingredients in food products. From probiotic production to storage and GIT delivery, bifidobacteria encounter a plethora of stresses. To cope with these environmental challenges, they need to protect themselves through stress-induced adaptive responses. We have determined the response of B. breve UCC2003 to various stresses (heat, osmotic, and solvent) using transcriptome analysis, DNA-protein interactions, and GusA reporter fusions, and we combined these with results from an in silico analysis. The integration of these results allowed the formulation of a model for an interacting regulatory network for stress response in B. breve UCC2003 where HspR controls the SOS response and the ClgR regulon, which in turn regulates and is regulated by HrcA. This model of an interacting regulatory network is believed to represent the paradigm for stress adaptation in bifidobacteria.

* Corresponding author. Mailing address: Alimentary Pharmabiotic Centre and Department of Microbiology, National University of Ireland, Cork, Ireland. Phone: 353 21 4901365. Fax: 353 21 4903101. E-mail: d.vansinderen{at}ucc.ie

{triangledown} Published ahead of print on 4 September 2009.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

{ddagger} Present address: Bio-Iliberis Research and Development, Edificio BIC, Parque Tecnológico de Ciencias de la Salud, E-18100 Armilla, Granada, Spain.

§ Present address: Department of Genetics, Biology of Microorganisms, Anthropology and Evolution, University of Parma, parco Area delle Scienze 11a, 43100 Parma, Italy.

Journal of Bacteriology, November 2009, p. 7039-7049, Vol. 191, No. 22
0021-9193/09/$08.00+0     doi:10.1128/JB.00897-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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